 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Anatomical Characterization and Protein Profiles in Adventitious and Storage Roots of Two Commercial Thai Cassava Varieties) ผู้เขียน: ศิริพร เวชกระจ่าง,  ดร.วิจารณ์ วิชชุกิจ, รองศาสตราจารย์ , Prachumporn Toonkool, ดร.สุตเขตต์ นาคะเสถียร, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractAnatomical changes and protein expression patterns were examined in adventitious and storage roots of two commercial varieties of cassava grown in Thailand, namely Rayong 1 (R1) and Kasetsart 50 (KU50). Both storage and adventitious roots were harvested consecutively every 7 days after planting for a period of 9 weeks. Adventitious and storage roots were collected separately, cross-sectioned to observe anatomical changes under light microscopy. Iodine-stain, indicating starch accumulation revealed that starch accumulation started 35 days after planting in both varieties. Total protein in adventitious and storage roots were extracted in ice-cold saline buffer and analyzed for protein profile using SDSPAGE. Similar to the anatomical changes, SDS-PAGE revealed unique protein bands in the cassava roots from 35 days after planting onward. Further analysis will be carried out to investigate and differentiate the unique protein patterns between adventitious roots and storage roots using 2-dimensional gel electrophoresis technique. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Nucleotide and Derived Amino Acid Sequences of the Cyanogenic Beta-Glucosidase (Linamarase) from Cassava (Manihot esculenta Crantz)) ผู้เขียน:Prachumporn Toonkool, Nusra Tongtubtim สื่อสิ่งพิมพ์:pdf AbstractMany isozymes of cassava cyanogenic ?-glucosidase (linamarase) exist, but only one cDNA sequence (pCAS5) has been reported thus far. In order to study the structure-function relationships in this enzyme, the cDNA of cassava linamarase was cloned and sequenced. In this report, six different cDNA sequences of linamarase from cassava were cloned by reverse transcription-polymerase chain reaction (RT-PCR) using primers designed from the sequence of pCAS5. Nucleotide sequences of all six clones showed 98-99% sequence identity to the nucleotide sequence of pCAS5. Derived amino acid sequences from four cDNA clones showed 98-99% sequence identity to that of pCAS5, while the other two cDNA clones contained nucleotide sequences that led to premature termination. |